Objective : The purpose of this research is to investigate the effects of Autogenic Training on autonomic nervous system for normal subjects with Heart Rate Variability(HRV). Then HRV of total subjects were analyzed by Sasang constitution, MBTI type and relaxation therapy experience. Method : Autogenic Training was applied on 39 subjects individually. Then HRV was examined each 5 minutes before and after Autogenic Training application. Results : 1. Mean HRV, SDNN, SDSD were significant different in HRV between before and after Autogenic Training application in total subjects. 2. SDNN, SDSD were significant different in HRV between before and after Autogenic Training application in Soeumin. Mean HRV, SDNN, SDSD were significant different in HRV between before and after Autogenic Training application in Taeeumin. Conclusion : The results suggest that Autogenic Training application have shown anti-stress effects. Autogenic Training application to Soeumin and Taeeumin have shown more anti -stress effects than application to Soyangin.