Objective: Recently biophysical research has shown very weak radiation of photons in plant, animal and human cells. Biophoton emission of the aliving system has received specific phenomena because of its scientific potential for monitoring metabolism to human body. This paper proposes a new challenge possibility of the biophoton emission in biophysiological human condition index. Method: Biophoton emission were measured with twenty one healthy volunteers. The study analyses existence for the effect of specific acupoint stimulus, exposure to the sun and low temperature in healthy volunteers. Two photomultiplier tubes which spectral range was from 300 nm to 650 nm was used for the detection of biophoton emission observation of possible acupuncture stimulation effect at left hand palm. Result: There was a reversion of relative emission rates from the palms affected the environment condition. Otherwise, when the acupuncture stimulation, the emission rate was not enough significantly change(p<0.05). Conclusion: In this study, biophoton emissions depend on the environment condition for all subjects. Measurments with the biophoton emission rate analysis might be much considered in future studies.