본 연구에서는 포도 가공 부산물인 포도씨를 이용하여 피부노화 및 색소침착에 관련된 tyrosinase 저해활성을 알아보고자 하였으며 폴리페놀 화합물과 총항산화력과의 상관관계를 비교·분석하고자 하였다. 포도씨 80% 메탄올 추출물은 hexane, chloroform, ethyl acetate, water 층의 순서로 분획 되었다. Tyrosinase 저해활성은 각 분획물보다 crude한 형태인 methanol 추출물의 효과가 더 높았으며 분획물 중 ethyl acetate 층이 가장 높은 tyrosinase 저해활성을 나타내었고 추출물과 분획물 모두 농도 의존적인 경향을 나타내었다. 포도씨 분획물 중 ethyl acetate층이 polyphenol과 flavonoid 함량이 가장 높았고 ABTS 라디칼 소거능 또한 가장 높은 결과를 나타내었으며 water 분획층도 비교적 높은 활성을 나타내었다. 본 연구 결과 tyrosinase 활성과 항산화 성분,항산화 활성 경향이 일치하는 것을 볼 수 있었으며 포도씨는 미백, 항노화 효과로 화장품 산업에서 천연 물질로 잠재적인 기능성을 가지고 있을 것으로 사료된다.
The objectives of this study were to determine inhibitory effect on tyrosinase activity and antioxidant activity of the methanolic extract from grape seeds and to investigate relationships between tyrosinase inhibitory activity and antioxidant activity of the extract. The 80% methanol extracts of grape seeds were fractionated subsequently with hexane, chloroform, ethyl acetate and water. The methanolic extract and fractions from grape seeds inhibited tyrosinase activity in a concentration dependent manner. The methanolic extracts showed the highest inhibitory effects on tyrosinase activity. The inhibitory effects of the ethyl acetate fraction from the methanolic extract on tyrosinase activity was higher than other fractions. The ethyl acetate fraction from methanolic extracts showed higher antioxidant activity and contained higher polyphenolic and flavonoid contents compared to other fractions. The correlation coefficients among the polyphenoilc content of methanolic extracts, ABTS radical cation scavenging activity and inhibitory effect of tyrosinase were relatively high. These results suggest that grape seeds may have potential as a depigmentation agent for cosmetics and functional food products.
