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Physicochemical Properties of Poly-γ-glutamic Acid Produced by a Novel Bacillus subtilis HA Isolated from Cheonggukjang
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  • Physicochemical Properties of Poly-γ-glutamic Acid Produced by a Novel Bacillus subtilis HA Isolated from Cheonggukjang
  • Physicochemical Properties of Poly-γ-glutamic Acid Produced by a Novel Bacillus subtilis HA Isolated from Cheonggukjang
저자명
Seo. Ji-Hyun,Kim. Chan-Shick,Lee. Sam-Pin
간행물명
Journal of food science and nutrition
권/호정보
2008년|13권 4호|pp.354-361 (8 pages)
발행정보
한국식품영양과학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A novel bacterium isolated from Cheonggukjang was identified as a glutamate-dependent Bacillus subtilis HA with 98.3% similarity to Bacillus subtilis Z99104. Optimization of poly-$gamma$-glutamic acid ($gamma$-PGA) production by modulating fermentation factors including carbon sources, nitrogen sources, inorganic salts and fermentation time was investigated. Optimum culture broth for $gamma$-PGA production consisted of 3% glutamate, 3% glucose and various salts, resulting in the PGA production of 22.5 g/L by shaking culture for 72 hr at $37^{circ}C$. Average molecular weight of $gamma$-PGA was determined to be 1,220 kDa through MALLS analysis. The $gamma$-PGA solution showed a typical pseudoplastic flow behavior, and a great decrease in consistency below pH 6.0 regardless of the same molecular weight of $gamma$-PGA. The molecular weights of isolated $gamma$-PGA were drastically decreased by heat treatment in various acidic conditions, resulting in different hydrolysis of $gamma$-PGA. The consistency of $gamma$-PGA solution was greatly decreased with increase heating time in acidic conditions.