The voltammetic behavior of Vitamin B6 (VB6) was studied at a glassy carbon electrode in phosphate buffers using cyclic, linear sweep and differential pulse voltammetry. The oxidation process was shown to be irreversible over the entire pH range studied (4.0-10.0) and was adsorption controlled. The adsorption amount of VB6 on the glassy carbon electrode was examined by chronocoulometry and the value of n, product of transfer coefficient and number of electrons transferred in the rate determining step, was determined from Tafel plot. VB6 was determined by differential pulse voltammetry and the peak current was found linearly with its concentration in the range of 3 10-7-2 10-4 mol L-1. The detection limit was 1 10-7 mol L-1. The procedure was successfully applied for the assay of VB6 in tablets.