Two different sensitivity classes of black cherry (Prunus serotina) under the natural growing environmental conditions were assessed adjacent to Air Monitoring Station located at Horton research center in Giles County, Virginia, USA. Ambient ozone concentrations, leaf gas exchange, and visible foliar injury were measured on-site during the growing seasons of 2000, 2001, and 2002. Ambient ozone exposures were sufficient to induce typical foliar visible injury corresponding with the reduction in photosynthetic activities only in sensitive black cherry. There were positive correlations between increasing cumulative ozone concentration and percent reduction in maximum net photosynthetic rates ($Pn_{MAX}$) under saturating light conditions and in quantum yield for carbon reduction (${Phi}CO_2$) of sensitive black cherry compared to tolerant black cherry. There was a negative correlation between chlorophyll content and percent leaf injury in sensitive black cherry. Furthermore, $Pn_{MAX}$ was inversely related to percent leaf injury.