There are many factors which affect the acoustical properties of a micro-speaker. Among the factors, the shape of the diaphragm is considered in this study. As an investigating method, the finite element methods and measurement techniques applied to study the acoustical properties according to diaphragm shape. In order to vary the stiffness of the diaphragm, the some patterns of comb teeth, such as the angle and the number of comb teeth, are applied to diaphragm. We can confirm that the change of the stiffness by the changing diaphragm shape affects the vibration and sound properties of the speaker. As a result, the reduction of the angle of the comb teeth increases the diaphragm stiffness and shifts the resonance frequency to a higher frequency range. The number of the comb teeth is related to the stiffness of the edge part.