Purpose: This study was conducted to test the effect of a 12-week exercise program on body composition. blood pressure, blood glucose, blood lipid and bone mineral density for middle-aged and aged women in rural areas. Methods: The subjects were 33 women at the age of 40-75. For statistical analysis, descriptive statistics and paired t-test were used with SPSSWIN 16.0. Results: The results were summarized as follows. First, body weight, BMI. % body fat. abdominal adipose, and waist circumference of the women decreased significantly after implementing the 12-week exercise program. Second, diastolic blood pressure, serum cholesterol, triglyceride, and LDL decreased significantly, and the HDL level increased significantly after the 12-week exercise program. Third, bone mineral density did not increase significantly after the program. Conclusion: These results suggest that the exercise program has an effect in decreasing body composition, and improving blood pressure, blood glucose, and blood lipid in middle-aged and aged women.