Objective : The aim of this research was to investigate the effect on five element constitutional acupuncture(Sa-am acupuncture) at 4 acupoints by needle manipulation to sedate Six Fu-Organ on hyperlipidemia induced by high fat diet in rats. Method : We recorded data of weight gain, food intake, food efficiency, weight of liver, heart, spleen, lung, kidney, levels of total cholesterol, triglyceride, HDL-cholesterol, AST, ALT in serum of rats fed high fat diet for 5 weeks. Spraque Dawley rats were divided into eight groups(normal; normal diet and non-acupuncture group, control; non-acupuncture group, Acu-LI; acupuncture at $SI_5{cdot}LI_5{cdot}BL_{66}{cdot}LI_2$ group, Acu-ST; acupuncture at $GB_{41}{cdot}ST_{43}{cdot}LI_1{cdot}ST_{45}$, Acu-SI; acupuncture at $BL_{66}{cdot}SI_2{cdot}ST_{36}{cdot}SI_8$, Acu-BL; acupuncture therapy at $ST_{36}{cdot}BL_{40}{cdot}GB_{41}{cdot}BL_{65}$, Acu-TE; acupuncture at $BL_{66}{cdot}TE_2{cdot}ST_{36}{cdot}TE_{10}$, Acu-GB; acupuncture at $LI_1{cdot}GB_{44}{cdot}SI_5{cdot}GB_{38}$. After needles inserted, it was rotated clockwise and anticlockwise 12 times on rats fed high fat diet. Results : The body weight was decreased in Acu-SI, Acu-TE, Acu-GB group, /the food intake was decreased in Acu-TE, / the food efficiency was decreased in Acu-SI, /serum triglyceride was decreased in Acu-LI, Acu-ST, Acu-BL, Acu-GB, /serum total cholesterol was decreased in Acu-LI, Acu-BL, serum ALP was decreased in Acu-LI, Acu-ST, Acu-BL, Acu-TE, Acu-GB. While the relative weight of heart was increased in Acu-LI, Acu-SI, the relative weight of kidney was increased in Acu-SI, Acu-GB. Conclusion : These results suggest that five element constitutional acupuncture(Sa-am acupuncture) may be having an therapeutic influence on hyperlipidemia induced by high fat diet. Further researches of acupuncture manipulation are needed in the future based on our study.