The Roll-to-Roll system including continuous flexible thin materials and roller has its wide range of applications especially in the electronic printing industry. The industry is growing rapidly and the printing speed is also improving. However, the printing machine based on web and roller system has it own problem. As the web speed increases, the failure to maintain the contact may occur and the air entrain between the roller and the paper web may exist. Air bubbles may remain attached to electronic ink on the web causing defects on product surface. With the development of image processing technique, the airflow around the web and rolls can be visualized and calculated by PIV method. In our experiment, the simple web and rolls system is used to R2R simulator. The flow field is studied at various web speeds and positions. The result shows that the flow field has complicated structure with turbulent characteristic and the main trend of flow is obtained by taking time average of flow field.