This paper describes microscale laser structuring of electrospun(ES) PCL and PET nanofiber meshes. Electrospinning produces non-woven meshes of synthetic or natural materials fibers with diameters ranging from micron down to the nanometer scales that are advantageous for the supporting the growth of the small scale structures. Ultrashort laser found to be effective on the fabrication of engineeredtissue scaffold with minimum heat affect and ultra precision ablation patterns. The affect of energy range for ablation quality was analyzed and ablation characteristics of PCL and PET were compared.