Two coumarins (1 and 6), one flavan-3-ol (2), one fatty acid (3), and two lignan glycosides (4 and 5) were isolated from the EtOAc and $CH_2Cl_2$ extract of the bark of Tilia amurensis. Their chemical structures were identified by comparing their physicochemical and spectral data with those of published in literatures. Compounds 4, 5, and 6 were isolated from Tilia genus for the first time. Compounds 2 and 3 showed potent inhibitory activity against both DNA topoisomerase I ($IC_{50}$ values; $49{mu}M$ and $4{mu}M$, respectively, with $18{mu}M$ of positive control compound, comptothecin) and DNA topoisomerase II ($IC_{50}$ values; $13{mu}M$ and $3{mu}M$, respectively, with $50{mu}M$ of positive control compound, etoposide). However, all compounds did not showed cytotoxicity against the human colon adenocarcinoma cell line (HT-29), the human breast adenocarcinoma cell line (MCF-7), and human liver hepatoblastoma cell line (HepG-2).