This paper presents real-time hybrid test method of large-scale MR damper applied to a building structure under seismic excitation. The real-time hybrid test using an actuator for the control performance evaluation of a MR damper controlling the response of earthquake-excited building structure is experimentally implemented. In the test, the building structure is used as a numerical part, on which a large-scale MR damper adopted as an experimental part was installed to reduce its response. At first, the force that is acting between a MR damper and building structure is measured from the load cell attached on the actuator system and is fed-back to the computer to control the motion of the actuator. Then, the actuator is so driven that the error between the interface displacement computed from the numerical building structure with the excitations of earthquake and the fed-back interface force and that measured from the actuator. The control efficiency of the MR damper used in this paper is experimentally confirmed by implementing this process of experiment on real-time.