This paper presents the backplane effects for two-wire transmission line with different line length crossing the rectangular aperture in an infinite ground backplane. The FDTD method is used to determine the characteristics of the backplane insertion loss and return loss of the transmission line in accordance with the transmission line spacing and additional wire lengths. The results show that the insertion gain is obtained for the narrow spacing of the transmission line and the insertion loss is appeared for the transmission line with the additional wire The measurements of return loss are performed to verify the theoretical analysis.