Sulfadiazine and silver sulfadiazine are well-known bactericidal agent routinely used clinical settings. Antimicrobial acid dyes were synthesized by introducing sulfadiazine or silver sulfadiazine and applied on nylon fabric. The Chemical Structure of the Synthesized dyes was identified by HPLC-mass. The dyeability of synthesized acid dyes for nylon fabric was similar to commercial acid dyes. Resistance to washing, rubbing and lightfastness were good. Nylon fabrics dyed with synthesized acid dyes had good antimicrobial properties. Durable antimicrobial properties after 20 times washing have shown good result that reduction ratio of colonies, is 99.9 %. Mixed dyeing were carried out using commercial acid dyes(leveling type) and synthesized dyes. The mixed dyeings have also shown good antimicrobial properties.