The cable which transfers the signal and power in an industrial robot has a problem of fatigue fracture like steel components. Since the cable is very flexible compared to other components of the system, it is difficult to estimate its motion numerically. Some studies have been done on a large deformation problem, especially in a cable, and a few attempts have been made to apply the absolute nodal coordinate formulation(ANCF), which can simulate a large deformation. Only researches about the fatigue life of structural cables or comparative studies of FEM and ANCF simulations can be found. This paper presents a method to simulate the behavior of the cable harness using the ANCF and to predict the fatigue life while computing the strain time history of the point of interest. Rigid body dynamics is applied for the robot system, while ANCF is used for the cable harness. The simulation is performed by using the dynamic analysis process. The material property of the cable is obtained by a test. A simplified model is prepared. With these data, the behavior of the cable is simulated and the fatigue life is predicted.