Objectives : The purpose of this study was to observe the effects of acupuncture and electro-acupuncture at $LR_3$ on intestinal motility in rat. Methods : We made over-activated state of intestinal motility with carbachol and suppressed state of intestinal motility with loperamide in rat and carried out needle retention acupuncture and high frequency electro-acupuncture at $LR_3$ in rat devided into pre-treatment group and post-treatment group. The charcoal travel rate was measured to evaluate the intestinal motility. Results : 1. High frequency electro-acupuncture at $LR_3$ have significant influences on intestinal motility of rat in normal state. 2. Pre-treatment of needle retention acupuncture, high frequency electro-acupuncture significantly decreased intestinal motility in rat which over-activated with carbachol. 3. Post-treatment of high frequency electro-acupuncture significantly increased intestinal motility in rat which suppressed with loperamide. Conclusions : These results suggest that acupuncture and electro-acupuncture at $LR_3$ are effective on intestinal motility in rat.