Although manual traction, one of pain therapies, was applied in clinic to relief pain, the study was rare on the manual force and displacement of ligaments at knee joint during manual traction. The aim of this study is to quantify not only manual force at knee joint but also elongated displacement of joint ligament by C-arm scanning and motion analysis. Twenty-one healthy subjects were tested with manual traction from grade I to grade III under neutral position by a physical therapist. We calculated traction force using joint farces of both hands and elongated displacement of joint ligament were measured. The results showed that traction forces by C-arm scanning analysis were averagely 1.67-fold greater than those by motion analysis, but elongated displacements were instead averagely 2.36-fold smaller than motion analysis. Finally, we could estimate relationship between traction force and elongated displacement at knee joint by two methods.