Induction of regeneration and maturation for the free-living gametophytes of Ecklonia cava Kjellman was conducted under various temperature, irradiance and photoperiod conditions. Culture conditions for female or male gametophyte fragments were 4 temperature (5, 10, 15 and $20^{circ}C$), 4 irradiance (5, 10, 20 and $40{mu}mol{cdot}m^{-2}{cdot}s^{-1}$) and 3 photoperiod (14:10, 12:12 and 10:14 h L:D). Female and male gametophyte fragments were maintained in active regeneration without reaching sexual maturity under $5{sim}10^{circ}C$, $5{sim}10{mu}mol{cdot}m-2{cdot}s-1$, 12:12h or 10:14h (L:D) and $15-20^{circ}C$, $20-40{mu}mol{cdot}m^{-2}{cdot}s^{-1}$, 14:10h (L:D), respectively. Sexual maturation of female and male gametophytes facilitated under $15^{circ}C$, $20-40{mu}mol{cdot}m^{-2}{cdot}s^{-1}$, 14:10h (L:D) and $5-10^{circ}C$, $5-10{mu}mol{cdot}m^{-2}{cdot}s^{-1}$, 10:14h (L:D), respectively. These results provide basic informations to control the regeneration or maturation of the free-living gametophytes for artificial seed production of E. cava.