The purpose of this study was to develop performance assessment materials of scientific inquiry skills on 62 scientific activities in elementary science textbook for third and fourth grades. For this study, a total of 318 elementary teachers were sampled and surveys were carried out to examine the status of performance assessment of scientific inquiry skills in school. The classification system of assessment methods by Hart(1994) was also analyzed to create assessment strategy of scientific inquiry skills for elementary school students. Based on the quantitative data analysis, literature study and existing performance assessment materials analysis, performance assessment materials were developed and applied to science classes for third and fourth grades. Teachers and students were interviewed after the classes for asking about merits and improvements and the performance assessment materials were revised.