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Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Community Profiles in the Rhizosphere of cry1AC-carrying Brassica rapa subsp. pekinensis
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  • Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Community Profiles in the Rhizosphere of cry1AC-carrying Brassica rapa subsp. pekinensis
  • Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Community Profiles in the Rhizosphere of cry1AC-carrying Brassica rapa subsp. pekinensis
저자명
Jung. Se-Ra,Park. Se-Mi,Kim. Dae-Ha,Kim. Seung-Bum
간행물명
The journal of microbiology
권/호정보
2008년|46권 1호|pp.12-15 (4 pages)
발행정보
한국미생물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The effect of genetically modified (GM) Brassica rapa subsp. pekinensis (Chinese cabbage) expressing Bt toxin gene (cry1AC) to the rhizosphere bacterial community was examined using the denaturing gradient gel electrophoresis (DGGE) fingerprinting method. From the visual comparison of the DGGE profiles, there were no significant differences between the profiles of Bt and control rhizosphere in both Suwon and Yesan samples. From the sequence analysis of the individual bands, Sphingomonas sp. of Alphaproteobacteria and several actinobacterial members were identified as the main bacterial taxa in both Suwon and Yesan samplesIn the multiple correspondence analysis, no clear separation between Bt and control rhizosphere was seen in both Suwon and Yesan datasets. The profiles of bulk soils were separated from those of rhizosphere. The DGGE fingerprinting analyses indicated that Bt crops did not significantly alter the genetic composition of rhizosphere bacterial communities.