Musculoskeletal disorder (MSD) problems have been increasingly reported in hospital sector but the problems were not addressed with respect to holistic aspects of the target population in Korea. Often, it is required to understand how MSD symptoms are associated with factors such as personal, work environmental and psychosocial stressors. To examine features of association between sets of MSD symptoms and the factors, a questionnaire survey was conducted in a university hospital. A 140-item questionnaire was developed and used for collecting information including factors (e.g., job/occupation, task/activity, job stress) and MSD symptoms. A total of 1,091 workers (male 23.7% and female 76.3%) were finally determined for data analyses. Prevalence rate for the whole body was 72% and, among body parts, the highest was 48.7% for the shoulder, followed by 34.6%(the low back), 32.7%(the leg/foot), 27.9%(the neck), 26.7%(the wrist) and 12%(the elbow). The symptoms were significantly different by job/occupational variable in each of all body parts except the neck. The symptoms were very significantly different by task/activity variables in each of all body parts while those symptoms were significantly different by psychosocial variables, depending on body part and gender. In the logistic regression analyses performed for MSD symptoms by body part and each of 3 factors, odds ratio values varied, ranging from 0.7 to 3.3. The controls for reducing the symptoms were discussed on the basis of the findings. The results show that the MSD symptoms can remarkably vary by the factors and, in particular, can be highly differential for the task/activity factor. This study suggests that MSD symptom features be examined by using various factors and then a higher differential factor be primarily utilized for controling MSD symptoms in general industry including hospital settings.