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A High-Efficiency Direct Somatic Embryogenesis System for Strawberry(Fragaria x ananassa Duch.) Cultivar Chandler
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  • A High-Efficiency Direct Somatic Embryogenesis System for Strawberry(Fragaria x ananassa Duch.) Cultivar Chandler
  • A High-Efficiency Direct Somatic Embryogenesis System for Strawberry(Fragaria x ananassa Duch.) Cultivar Chandler
저자명
Husaini. Amjad M.,Aquil. Samina,Bhat. Mukhtar,Qadri. Tabassum,Kamaluddin. Kamaluddin,Abdin. Malik Zainul
간행물명
Journal of crop science and biotechnology
권/호정보
2008년|11권 2호|pp.107-110 (4 pages)
발행정보
한국작물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A high-efficiency, reproducible somatic embryogenesis system for strawberry cultivar Chandler was developed. Thirty-one somatic embryos per explant(max no.) were recorded in leaf discs which were cultured on medium containing MS salts+$B_5$ vitamins+2% glucose+4.0 mg $1^{-1}$TDZ(Thidiazuron) and incubated at $10{pm}1^{circ}C$ under darkness for one week followed by three weeks under 16-h photoperiod. The scanning electron microscopic(SEM) ontogeny revealed the normal development of somatic embryos from globular to heart-shaped and dissection microscopy from torpedo-shaped to cotyledonary-stage embryos. The maximum germination percentage of 48% could be obtained on MS medium containing kinetin(1.0 mg $1^{-1}$) and the maximum survival percentage(79%) of plantlets after four weeks was found to be in the mixture of vermiculite, peatmoss, and soilrite(1:1:1).