Biological nutrient removal (BNR) sludge was added to a sequencing batch reactor (SBR) in the addition ratios of 0%, 20%, 40%, 50% while observing the variation of nutrient removal characteristics and microorganism groups. When the BNR sludge was added in a ratio over 40%, the characteristics of EBPR (enhanced biological phosphorus removal) was shown at the 27 days. However, a distinct BNR was not shown when the addition ratio of BNR sludge was lower than 40%. The organic removal efficiency were shown as 90% in all SBRs irrespective of the addition ratio of BNR sludge. At the 27 days, the phosphorus removal efficiencies were shown as 40%, 55%, 77% and 69%, respectively, according to the addition ratio of BNR sludge. Overall, efficient nitrification and phosphorus removal was shown when the added BNR sludge ratio was over 40%.