This study was carried out to investigate the anti-hypercholesterolemic effects of Wasabia japonica (WJ) in hypercholesterolemic rats. Sprague-Dawley male rats were randomly assigned to one normal diet and three high cholesterol diet groups which contained 1% (w/w) cholesterol. After 4 weeks cholesterol diet, the groups of high cholesterol diet were classified to control (normal diet only), WJR5 (normal diet plus 5% WJ root) and WJL5 (normal diet plus 5% WJ leaf). There were no difference in the body weight gain, food intake and Food efficacy ratio (FER). The liver and kidney weights change of high cholesterol diet groups were higher than the normal diet group (p<0.05), but there was no significant difference of change between control and WJ diet groups. Concentration of serum HDL-cholesterol were significantly increased in WJ diet groups compared with control group (p<0.05). Concentration of serum LDL-cholesterol were significantly decreased in the groups of WJR5 diet compared with control group (p<0.05). The results of the study indicate that the diet of WJ can ameliorate the status of HDL-cholesterol and LDL-cholesterol in high cholesterol diet rats. In view of these results, WJ is expected to be an effective material for the anti-hypercholesterolemic in high cholesterol diet rats.