This paper deals with speed control of DC servo motor using a Fuzzy tuning observer. Speed sensor detect a speed of rotor continuously. But it have a limit as a driving speed to detect speed precisely. So it is problem to improve the performance of the driving system. To solve the problem, it is studied to detect a speed of DC motor without sensor. In particular, study on the method to estimate the speed using the observer is performed a lot. In this parer, the gain of the observer is properly set up using the fuzzy observer. The fuzzy observer has a superior transient characteristic and is easy to implement compared the existing method is designed. It estimate the derivative of the armature current directly using the armature current measured in the DC motor. It estimate the speed of the rotor using the differentiation. It is proposed speed sensorless control method using the estimated speed. Optimal gain of Luenberger observer is set up using the fuzzy observer and adapted speed control of DC servo motor low speed operation. It is proved excellence and feasibility of the presented observer from the comparison tested a case with a speed sensor and a case without a speed sensor which used a highly efficient drive and 200W DC servo motor starting system.