Analytical method to analyze the tolerance effect on the vehicle ride comfort is suggested in this paper. Ride comfort is one of the most important performance indices which decide the vehicle design quality. In general, the ride comfort is affected by the variations of parameters of a vehicle model. Therefore, the effects of the parameters on the ride comfort need to be evaluated statistically based on the whole-body vibration of the vehicle. In this paper, weighted RMS values of the acceleration PSD of a seat position are used to define the ride comfort. The equations of motion and the sensitivity equations are derived based on a 5-DOF vehicle model. By employing the sensitivity information of the acceleration at the seat position, the tolerance effect on the vehicle ride comfort could be effectively analyzed.