The occurrence of longitudinal cracking in concrete deck of box girder bridge is affected by many factors, but the most important factors are the shrinkage and thermal gradient of deck slabs. In this study, therefore, the tensile stresses at the bottom of deck were calculated from the experimental data(autogeneous shrinkage, drying shrinkage, and thermal gradient of deck slab). Also, the possibility of longitudinal cracks at bottom of deck was estimated. For this purpose, full-scale box girder segments have been fabricated and tested. The thermal gradients and shrinkage strains of deck slabs were measured after placement of concrete. Also, analytic program was conducted for the evaluation of longitudinal cracking in bridge deck considering differential shrinkage induced from non-uniform moisture distributions in concrete.