Purpose: We investigated the effects of physical practice, mental practice, and cross education using serial reaction time (SRT). We recruited 21 right-handed healthy males and females who gave consent and had no clinical history for their upper limbs. Methods: The subjects were divided into three groups; actual practice (n=7), mental practice (n=7), and controls (n=7), who performed actual training, mental training, or no intervention respectively for three weeks. Super lab 4.0 displayed four symbols on the monitor and subjects pushed on the matching button, with reaction time assessed pre- and post-intervention. Results: Reaction time was significantly lower after actual or mental practice (p<0.05). Actual practice also decreased left hand reaction time. Conclusion: Actual practice and mental practice can improve motor learning, but mental practice is not sufficient for cross education.