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Identification and Characterization of a New Strain of the Unicellular Green Alga Dunaliella salina (Teod.) from Korea
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  • Identification and Characterization of a New Strain of the Unicellular Green Alga Dunaliella salina (Teod.) from Korea
  • Identification and Characterization of a New Strain of the Unicellular Green Alga Dunaliella salina (Teod.) from Korea
저자명
Polle. Jurgen E.W.,Struwe. Lena,Jin. Eon-Seon
간행물명
Journal of microbiology and biotechnology
권/호정보
2008년|18권 5호|pp.821-827 (7 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The unicellular green alga Dunaliella salina is a halotolerant eukaryotic organism. Its halophytic properties provide an important advantage for open pond mass cultivation, since D. salina can be grown selectively. D. salina was originally described by E. C. Teodoresco in 1905. Since that time, numerous isolates of D. salina have been identified from hypersaline environments on different continents. The new Dunaliella strain used for this study was isolated from the salt farm area of the west coastal side of South Korea. Cells of the new strain were approximately oval- or pear-shaped (approximately $16-24;{mu}m$ long and $10-15;{mu}m$ wide), and contained one pyrenoid, cytoplasmatic granules, and no visible eyespot. Although levels of $eta$-carotene per cell were relatively low in cells grown at salinities between 0.5 to 2.5 M NaCl, cells grown at 4.5 M NaCl contained about a ten-fold increase in cellular levels of $eta$-carotene, which demonstrated that cells of the new Korean strain of Dunaliella can overaccumulate $eta$-carotene in response to salt stress. Analysis of the ITS1 and ITS2 regions of the new Korean isolate showed that it is in the same clade as D. salina. Consequently, based on comparative cell morphology, biochemistry, and molecular phylogeny, the new Dunaliella isolate from South Korea was classified as D. salina KCTC10654BP.