The natural dyeing of cotton fabric with Perilla frutescens var. acuta extract was investigated. The proper extraction temperature and time were $100^{circ}C$ and 120 minutes. The proper temperature, time and colorant powder concentration for the dyeing of cotton fabric with Perilla frutescens var. acuta powder were $90^{circ}C$, 60 minutes and 15%(o.w.b.), respectively. In various mordanted methods, the K/S values of simultaneous-mordanted methods were higher than those of pre-and post-mordanted methods. And the Cu-and Fe mordant showed higher K/S values than those of other mordants. Light colorfasness of mordanted cotton fabric was poor, but the other colorfasnesses were shown to be good. The cotton fabric mordanted with Cu mordant was showed effective bacterial reduction.