Pine needle (Pinus densiflora Sieb. et Zucc.) extract has been used to improve cardiovascular disorders, detoxification of nicotine, the infirmities of age and curing diseases of unidentified symptoms in folk medicine. To determine the facts behind the traditional belief, we tried to investigate the effects of fresh and self-fermented pine needle extracts of different aging. Fibrinolytic activities of the extract indicated that activity depends on time and also with aging of the product. It was also found that the extract can lower the blood plasma cholesterol and triglyceride in cholesterol fed rat. Also, Self-Fermented Pine Needle Extracts 7 years old (SFPE 7) ($200;{mu}g/mL$) reduce the frequency and amplitude of pacemaker currents in Interstitial Cells of Cajal (ICC) of murine small intestine by modulating ATP-sensitive potassium channels. Therefore, the investigation indicated that self-fermentation improves efficacy of the pine needle extracts reducing risk of cardio-vascular related disorders and would be an important source in nutraceutics.