Objectives : The aim of this study is to investigate the possibility of acupuncture to influence thyroglossal cyst. Methods : Acupuncture treatment was applied on several acupoints in the afternoon three to four times a week for 12 weeks. The effect of acupuncture treatment was evaluated with Visual Analogue Scale(VAS) and THYROID USG manipulated by a specialist in radiology. Results : VAS was decreased from 10 to 2, and this effect occurred in the first half of the treatment period. THYROID USG scan has shown that acupuncture treatment reduced the depth of the cyst approximately 6 ㎜ in the first half of the period, but not in the latter half. Conclusions : From this study, we suggest that acupuncture may take part in the treatment of thyroglossal cyst.