Purpose: This study was performed to determine anti-imflammatory effects of Haedoksamultang. Methods: In this study, I examined the effects of Haedoksamultang on the production of nitric oxide(NO), tumor necrosis factor-${alpha}$(TNF-${alpha}$), and interlukin-1${eta}$(IL-1${eta}$) as well as the expression of inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), TNF-${alpha}$, and IL-1${eta}$ in RAW 264.7 cells. Haedoksamultang inhibited LPS-stimulated NO production. Western blotting and RT-PCR analysis showed that Haedoksamultang suppressed LPS-induced iNOS and COX-2 protein and mRNA expression in RAW 264.7 cells. Haedoksamultang also suppressed the expression and production of LPS-stimulated TNF-${alpha}$ and IL-1${eta}$ in RAW 264.7 cells. Haedoksamultang inhibited NF-${kappa}B$ activation in LPS-treated RAW 264.7 cells. Moreover, this compound blocked $I{kappa}B-{alpha}$ phosphorylation and nuclear translocation of the cytosolic NF-${kappa}B$ p65 subunit, which highly correlated with the production and expression of inflammatory mediators. Results: Haedoksamultang suppresses that inflammation-associated gene expression by blocking NF-${kappa}B$ activation. Conclusion: These results suggest that Haedoksamultang may be beneficial for treating inflammatory disease.