브로콜리의 새싹 발아와 생리활성에 효과적인 영향을 미치는 LED 광질 종류를 구명하기 위해 청색, 녹색, 적색, 백색, 황색, 적색+청색광을 조광 14시간, 암조건 10시간, 온도는 주간 25, 야간 18로 조절하여 종자 발아와 새싹을 생장을 시켰다. 생리활성 조사는 새싹을 메탄올로 추출한 것을 이용하여 실시하였다. 종자 발아율은 광의 종류에 관계없이 3일 만에 100%의 발아율을 나타냈다. 파종 후 8일째의 신선중은 청색광처리구에서 0.389g/10plants로 가장 높았다. 총페놀화합물 함량은 백색광 처리구에서 $83.0mg{cdot}L^{-1}$으로, 총플라보노이드 함량은 청색광 처리구에서 $72,6mg{cdot}L^{-1}$로 가장 많았다. 전자공여능은 추출물 $2,000mg{cdot}L^{-1}$일 때 백색광 처리구에서 93.5%로 가장 높게 나타났다. 아질산염 소거능은 추출물이 $500mg{cdot}L^{-1}$일 때 황색광 처리구에서 66.9%로 가장 높게 나타났다 Tyrosinase 저해 활성은 추출물이 $2,000mg{cdot}L^{-1}$일 때 적색광 처리구에서 14.5%로 가장 높게 나타났다.
This study was carried out to investigate into the effect of light-emitting diode (LED) for the light quality as a light source on the broccoli seed germination and the physiological activity of vegetable sprouts. We have also germinated seeds of the broccoli and applied LED as a light quality such as blue, green, red, white, yellow and red + blue color lights to their sprouts for 14 hours and kept dark for 10 hours at the temperature of $25^{circ}C$ (day)/$18^{circ}C$ (night). Broccoli sprouts were extracted by methanol and their physiological activities were examined. All broccoli seeds were germinated at 3 days after seeding regardless of the light color. Total sprout fresh weight were mostly became highest by 0.389g (10 plants) at 8 days after seeding when their sprouts were grown under blue color light. Total phenol compound contents in broccoli sprouts were extremely increased by $83.0;mg{cdot}L^{-1}$ under the white light, and total flavonoid contents were most much more by $72.6;mg{cdot}L^{-1}$ under the blue light. DPPH radical scavenging activity at $2,000;mg{cdot}L^{-1}$ were most highest by 93.5% in broccoli sprouts grown under the white light. Nitrite radical scavenging activity at the concentration of $500;mg{cdot}L^{-1}$ in sprout extracts were the most increased by 66.9% under the yellow light, and tyrosinase inhibition activity at $2,000;mg{cdot}L^{-1}$ in sprout extracts were by 14.5% under red light.
