In spite of rapidly increasing interests in digital manufacturing, there still lacks of a systematic approach in manufacturing line flow analysis via modeling and simulation; currently, the parameters for designing manufacturing line are defined by being solely based on engineers experiences. The paper proposes an application of the genetic algorithm to a discrete event line simulation finding optimal set of parameters for manufacturing line balancing problem. The proposed method has been applied to two example problems-one is a simple manufacturing model and the other for shipyard industry-in order to demonstrate its validity and usefulness.