This study was intended to analyze difference of vocal folds movements between upon wakeup and in several hours later in the morning. The difference of vocal fold movements was compared with fundamental frequency and a range of fundamental frequencies from maximum to minimum. The participants were 30 female adults between 20 and 29 years old. Voice samples were collected from their reading sentence (Jeong, 1993). The first sampling was conducted within 5 minutes after wakeup, while the second on 1 hour after the first sampling. Finally, the third voice sample was collected on 6 hours after the second sampling. The results of this study were as follows: First, fundamental frequency of the participants were by hour significantly time-dependent(F=7.843). Post-hoc multiple comparison (LSD) was conducted to determine when the difference could be observed. The result showed significant differences between upon wakeup and 6 hours later (p< .001) and between 1 hour later and 6 hours later (p< .05). Second, there were a significantly time-dependent ranges of fundamental frequencies of participants by hour (F=3.130). According to the results of the LSD analysis the significant differences in range of fundamental frequencies were found between upon wakeup and 1 hour later and also between wakeup and 6 hours later (p< .05). The results above indicate that vocal fold movements upon wakeup is different from those of several hours later.