This study was carried out to investigate the prevalence of fascioliasis of slaughtered and farmed cattle in Gwangju area from February to November in 2007. A total of 1,000 cattle fecal samples were collected from slaughter houses (n=805) and farms (n=185). Twelve (1.2%) were found as positive cases with excretion of the egg of Fasciola spp in the fecal specimens, and 128 (12.8%) were positive in intestinal parasitism using the flotation and sedimentation procedures. The infection rate of fluke larvae from the slaughtered cattle at abattoirs in Gwangju was 0.75% (6 out of 805 heads). In histopathology, there were several liver lesions such as inflammation with infiltration of eosinophil, polymorphonuclear cells, mononuclear cells and multinucleated giant cells, proliferation of connective tissue, calcification and abscess formation.