Cruciferous hairy roots are often used for improving drought adaptability, peroxidase production, and in vitro subculturing of Plasmodiophora brassicae. For metabolic engineering, Agrobacterium tumefaciens-mediated systems have previously been developed for hairy root production in other plant species. Here, we used the rolABC gene binary construct in A. tumefaciens strain GV3101 to establish cultures of Chinese cabbage hairy roots. On both solid and liquid media, the rolABC hairy root lines exhibited a wild-type hairy root syndrome in terms of their growth and morphology. This demonstrates that those three genes are sufficient to induce high-quality hairy roots in Chinese cabbage. Such a system could be useful for the stable production of secondary metabolites in that species.