Objective: To investigate the changes of facial temperature by Miso Facial Rejuvenation Acupuncture treatment. Methods: One middle-aged women who has no other disease was recruited. The Miso Facial Rejuvenation Acupuncture was performed on only right face. We measured the facial temperature using the Digital Infrared Thermal Imaging (DOREX Inc. Spectrum 9000 MB, USA, D.I.T.I.) before, immediate after and 10 minutes after treatment. And we used the Wilcoxon signed rank test (P<0.05) to compare the difference of facial temperature at each time. Results: Right facial temperature (the area treated by acpuncuture) increased immediately from $30.02{pm}1.87^{circ}C$ to $32.24{pm}1.03^{circ}C$, the change of temperature is statistically significant. At 10 minutes after treatment, right facial temperature decreased little, but there was no statistical significance. Left facial temperature increased little, but there was no statistical significance. The difference between right and left face increased after Miso facial rejuvenation acupuncture treatment. Conclusion: Miso facial rejuvenation acupuncture could make the facial temperature increase.