1. Objectives The purpose of this study is to investigate the anti-aging and anti-oxidant effects of Hyeongbangjiwhang-tang decoction(HGD) in SD rats. 2. Methods This experiment was used the tissue of kidney, bladder and spleen cells of 6, 52 and 68 weeks old SD rats. Each age group was again divided into three groups. One group, as normal group, was not-treated cells, another group, as control group, was saline-treated cells, and the last group, as experimental group, was HG-treated cells. After culture for 48 hours, each groups measured the level of SOD, GSH, MDA and NO in the tissue of kidney, bladder and spleen cells. 3. Results and Conclusions The activity of SOD were significantly increased in kidney cell of 68 w-HGD group, bladder cell of 52 w-HGD group and in spleen cell of 52,68 w-HGD group compared with those of the control groups. The level of GSH were significantly increased in bladder cell of 52w-HGD group and in spleen cell of 68w-HGD group compared with those of the control groups. The level of MDA were significantly decreased in bladder cell of 68 w-HGD group and in spleen cell of 68w-HGD group compared with those of the control groups. The level of NO were significantly decreased in kidney cell of 68 w-HGD group, bladder cell of 52, 68w-HGD groups, and in spleen cell of 68w-HGD group compared with those of the control groups. These results suggest that HGD(Hyeongbangjiwhang-tang) has anti-oxidant effects in aged rats.