Objectives : The aim of this study is to collect data for toxicity and safety of Mud chrysanthemum indicum. Methods : In this study, we investigated the acute toxicity for buthanol-extracted Mud schrysanthemum indicum, 25 male and 25 female mice were observed for 14days after one day oral administration of Mud chrysanthemum indicum at the respective doses of 0(control group), 1024, 1280, 1600 and 2000mg/kg. Results : We observed survival rates, general toxicity, change of body weight and autopsy. No abnormality was found for all cases. Conclusions : The data confirmed that Mud chrysanthemum indicum is free from, the toxicity and safety problems. Compared with the control group, we could not find any toxic alteration in all treated groups (1024, 1280, 1600 and 2000mg/kg), In conclusion, LD50 of Mud chrysanthemum indicum was over 2000 mg/kg and it is very safe to mice.