Objectives : The purpose of this study is to compare the effects of the acupuncture at affected limb and unaffected limb on treating ankle sprain of acute stage. Methods : From October 1st, 2007 to March 23rd, 2008, the 60 patients who had visited Dongshin mokdong oriental medical hospital and Gwangju oriental medical hospital, Dongshin university with acute ankle sprain were divided into 2 groups ; one group took acupuncture at affected limb, and the other group took acupuncture at unaffected limb. To evaluating the efficiency of each treatment, Visual Analog Scale(VAS) and Ankle-Hindfoot Scale(AHS) were applied before treatment and after 3rd treatment. Results : As a result of evaluating by using VAS, the score of acupuncture at unaffected limb was marked lower than the score of acupuncture at affected limb. As a result of evaluating by using AHS, the score comparison between the two groups had no significance after treatment. Conclusions : Acupuncture at unaffected limb is more effective than acupuncture at affected limb on treating ankle sprain of acute stage.