The objective of this study was to investigate the spray characteristics of commercial biodiesel fuel at various fuel injection conditions. To examine the effect of various factors on the development of a biodiesel sprays, experiments were conducted at the various injection pressures, ambient pressures and blending ratio of bio-diesel fuels. As a result of experimental study, it was shown that the increase of blending ratio of biodiesel had little influence on spray behaviors under applied fuel injection condition in this study. Because macroscopic characteristics of biodiesel-blended diesel fuel were almost same as that of petrodiesel fuels, it was found that the commercial biodisel is applicable to conventional diesel engine.