This paper deals with the dynamic characteristics of the shaft with impeller model which is the most important part in developing the resin mixing machine. Through reverse engineering, it is possible to make the shaft with impeller geometry model which is necessary vibration characteristic analysis by commercial impeller. The natural frequency analysis and structural analysis using finite element analysis software are performed on the imported commercial shaft with impeller model. The most important fundamental natural frequency of the shaft with impeller model is around 14.5 Hz, which well agrees with modal testing. The most effective design variables were extracted by ANOM(analysis of means) and pareto chart. This paper presents approximation 2nd order polynomial as design variables using RSM(response surface methodology). Generally, RSM take 2 or 3 design variables, but this method uses 5 design variables with table of mixed orthogonal array. Further more, the analyzed result of the commercial shaft with impeller is to be utilized for the structural design of resin chock mixing machine.