The purpose of this study was to develope the education program using multimedia for endocrine disruptor, and to examine the learning effects of it on high school students. The subjects were 70 tenth grade students in the academic high school located in Chungnam. With dividing them into two groups: the controlled group and the experimental one, lessons were executed respectively. Total average of achievement was significantly different between control and experimental groups. Using multimedia learning can be effective in improving in terms of knowledge achievement. Meaningful difference did appear from almost, but did not appear between using multimedia materials group and traditional approach to tuition classes. The most of experimental group students were interesting of the education program using multimedia, and thought that multimedia learning materials were helpful to understand the lesson about endocrine disruptor. And experimental group students were more active and positive in the lesson using multimedia than in the traditional ones. By providing students with the proper learning materials that go with their traits and preference, the educational environments that students can take part in the class more positively and actively should be provided.