To improve mating rate of the bumblebee, Bombus terrestris, temperature, photoperiod and illumination during mating periods favorable for B. terrestris were investigated. The mating rate of queen mated at $19^{circ}C$ was 92.1%, which was 2.1-5.9% higher than that of $22^{circ}C$ and $25^{circ}C$. $19^{circ}C$ was more effective than at $22^{circ}C$ and $25^{circ}C$ in death rate during mating periods. The survival rate after hibernation of queen mated at $19^{circ}C$ was 3.0-17.7% higher than that of $22^{circ}C$ and $25^{circ}C$. At the photoperiod regimes during mating periods, queen mated at 14 L was more effective than 12 L and 16 L in death rate during mating, survival rate after hibernation, and egg-characteristics. In case of illumination during mating periods, intensity of over 1000 lux was suitable for mating B. terrestris queen in colony development. Therefore, we supposed that mating temperature favorable for B. terrestris was $19^{circ}C$ and photoperiod for mating was 14 L, and illumination was over 1000 lux.