A fast, simple, inexpensive, and reproducible sample preparation for extraction and analysis of HD in water was studied using single-drop microextraction(SDME) and GC-MS. Operation parameters, such as extraction solvent, extraction time were optimized. The optimized conditions were $1{mu}L$ trichloroethylene and 10 min extraction time. In these conditions, about 42 times higher enrichment factor(EF) was obtained. The detection limit of HD was $1{mu}g/L$, and the precision expressed as relative standard deviation was about 9.0%.