19 Aromatic ring and L-amino acid ester contained rupestonic acid amide derivatives 2a~2l, 3a~3g were synthesized and preliminarily evaluated in vitro against influenza virus $A_3$,B and herpes simplex virus type 1 (HSV-1), 2(HSV-2) by the national center for drug screening of China. The rusults showed that 2i possessed the highest inhibition against both influenza virus $A_3;(TC_{50};=;120.6;{mu}mol/L,;IC_{50}=;19.2;{mu}$mol/L, SI = 6.3) and B (T$C_{50};=;120.6;{mu}mol/L,;IC_{50}=;29.9;{mu}$mol/L, SI = 4.0); 2g was more active against influenza $A_3$ virus at very low cytotoxicity ($TC_{50};>;2092.1;{mu}mol/L,;IC_{50}=;143.7;{mu}mol/L,$ SI > 14.6) than the parent compound; Compounds 2b, 2c, 2f showed higher activities both against HSV-1 and HSV-2 than that of the parent compound, and 2f was the most potent inhibitor of HSV-1 ($TC_{50};=;200.0;{mu}mol/L,;IC_{50};=;11.3;{mu}mol$/L, SI = 17.7 ) and HSV-2 ($TC_{50};=;200.0;{mu}mol/L,;IC_{50};=;20.7;{mu}mol$/L , SI = 9.7).