This study was to develope the functional work clothing for livestock farmers. Major demanding performances for livestock work clothing are anti-soil and anti-bacterial properties. On surveys, functional fabrics that have anti-soil, anti-bacterial and waterrepellent performances were developed and the work clothing that have adaptability to body movements were manufactured. The designs of livestock working clothes were two piece and one piece with rubber bade in waist. The thermal responses of subjects wearing the winter working clothes for stock farming worker were measured in the climate chamber($17^{circ}C$, 40% R.H.). The main results were summarized as follows: Total body weight loss was smaller and the mean skin temperature was higher in developed clothes than the market product. Clothing micro-climate of developed clothes was lower than the market clothes. Subjective sensation did not have significant differences. From the results of various evaluation, developed garments for livestock workers showed better efficiency than the market product.